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cluster differentiation (cd) 14 microbeads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cluster differentiation (cd) 14 microbeads
    Cluster Differentiation (Cd) 14 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cluster+differentiation+(cd)+14+microbeads/cd14/ppr0712543-82-19-23
    Average 90 stars, based on 1 article reviews
    cluster differentiation (cd) 14 microbeads - by Bioz Stars, 2026-10
    90/100 stars

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    Cell Culture:

    Article Title: Impact of JAK / STAT inhibitors on human monocyte‐derived‐macrophages stimulated by cigarette smoke extract and lipopolysaccharide
    Article Snippet: .. The PMBC were then sorted using the human cluster of differentiation (CD)‐14 magnetic microbeads separation kit (Miltenyi Biotec) and resuspended in cell culture medium. .. The medium was constituted of Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco, Life Technologies), supplemented with 1% of l ‐Glutamine (Gibco, Life Technologies), 0.1% Penicillin–Streptomycin (Gibco, Life Technologies) and 10% fetal bovine serum (Hyclone, Fisher Scientific).

    other:

    Article Title: Investigating the immunological function of alpha-2-glycoprotein 1, zinc-binding in regulating tumor response in the breast cancer microenvironment
    Article Snippet: The cell suspension was treated with a FcR blocking 136 reagent (Miltenyi Biotech, Bergisch, Gladbach, Germany) and Cluster of Differentiation (CD) 14 MicroBeads (Miltenyi 137 Biotech, Bergisch, Gladbach, Germany) for 15 min at 4°C.

    Article Title: Tonabersat suppresses priming/activation of the NOD-like receptor protein-3 (NLRP3) inflammasome and decreases renal tubular epithelial-to-macrophage crosstalk in a model of diabetic kidney disease
    Article Snippet: Primary human monocytes were isolated from PBMCs using cluster of differentiation-(CD)14 microbeads (Miltenyi Biotec, Germany) following manufacturer’s instructions.

    Incubation:

    Article Title: 14-Day Ketone Supplementation Lowers Glucose and Improves Vascular Function in Obesity: A Randomized Crossover Trial.
    Article Snippet: .. Conjugated antibody specific for human cluster of differentiation (CD)14-Vioblue (Clone REA599; Miltenyi Biotec) and CD45-APCVio770 (Clone REA747; Miltenyi Biotec) were added to each culture and incubated for 10 minutes. .. Red blood cell lysis buffer (Miltenyi Biotec; 1× final concentration) was then added to the samples, which were lysed for 15 minutes in the dark at room temperature.

    Isolation:

    Article Title: HIVEP1 Is a Negative Regulator of NF-κB That Inhibits Systemic Inflammation in Sepsis.
    Article Snippet: Buffy coats were collected from heparin anticoagulated blood by density-gradient centrifugation using Ficoll-paque plus (GE Healthcare, Stockholm, Sweden). .. Subsequently, monocytes were isolated using cluster-of-differentiation (CD)14+ magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany, # 130-050- 201) and MACS LS columns (Miltenyl Biotech). .. For stimulations, monocytes were placed in a 48-well plate (106 per well; Greiner Bio-One, Frickenhausen, Germany) in RPMI1640 medium containing 10% fetal calf serum (FCS),100 U/ml penicillin, 2 mM L-glutamine and stimulated with medium, Pam3CSK4 (1 μg/mL; Invivogen, San Diego, CA) or LPS (from Escherichia coli O111:B4;100 ng/mL; Invivogen) for 4 hours.

    Article Title: Tonabersat suppresses priming/activation of the NOD-like receptor protein-3 (NLRP3) inflammasome and decreases renal tubular epithelial-to-macrophage crosstalk in a model of diabetic kidney disease.
    Article Snippet: Isolated PBMCs were then resuspended and cultured in Gibco RPMI-1640 media (Fisher Scientific, US) with 10% foetal calf serum (FCS). .. Differentiation of human primary monocytes to macrophages Primary human monocytes were isolated from PBMCs using cluster of differentiation-(CD)14 microbeads (Miltenyi Biotec, Germany) following manufacturer’s instructions. ..

    Article Title: HIVEP1 Is a Negative Regulator of NF-κB That Inhibits Systemic Inflammation in Sepsis
    Article Snippet: Buffy coats were collected from heparin anticoagulated blood by density-gradient centrifugation using Ficoll-paque plus (GE Healthcare, Stockholm, Sweden). .. Subsequently, monocytes were isolated using cluster-of-differentiation (CD)14 + magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany, # 130-050-201) and MACS LS columns (Miltenyl Biotech). .. For stimulations, monocytes were placed in a 48-well plate (10 6 per well; Greiner Bio-One, Frickenhausen, Germany) in RPMI1640 medium containing 10% fetal calf serum (FCS),100 U/ml penicillin, 2 mM L-glutamine and stimulated with medium, Pam3CSK4 (1 μg/mL; Invivogen, San Diego, CA) or LPS (from Escherichia coli O111:B4;100 ng/mL; Invivogen) for 4 hours.

    Magnetic Beads:

    Article Title: HIVEP1 Is a Negative Regulator of NF-κB That Inhibits Systemic Inflammation in Sepsis.
    Article Snippet: Buffy coats were collected from heparin anticoagulated blood by density-gradient centrifugation using Ficoll-paque plus (GE Healthcare, Stockholm, Sweden). .. Subsequently, monocytes were isolated using cluster-of-differentiation (CD)14+ magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany, # 130-050- 201) and MACS LS columns (Miltenyl Biotech). .. For stimulations, monocytes were placed in a 48-well plate (106 per well; Greiner Bio-One, Frickenhausen, Germany) in RPMI1640 medium containing 10% fetal calf serum (FCS),100 U/ml penicillin, 2 mM L-glutamine and stimulated with medium, Pam3CSK4 (1 μg/mL; Invivogen, San Diego, CA) or LPS (from Escherichia coli O111:B4;100 ng/mL; Invivogen) for 4 hours.

    Article Title: HIVEP1 Is a Negative Regulator of NF-κB That Inhibits Systemic Inflammation in Sepsis
    Article Snippet: Buffy coats were collected from heparin anticoagulated blood by density-gradient centrifugation using Ficoll-paque plus (GE Healthcare, Stockholm, Sweden). .. Subsequently, monocytes were isolated using cluster-of-differentiation (CD)14 + magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany, # 130-050-201) and MACS LS columns (Miltenyl Biotech). .. For stimulations, monocytes were placed in a 48-well plate (10 6 per well; Greiner Bio-One, Frickenhausen, Germany) in RPMI1640 medium containing 10% fetal calf serum (FCS),100 U/ml penicillin, 2 mM L-glutamine and stimulated with medium, Pam3CSK4 (1 μg/mL; Invivogen, San Diego, CA) or LPS (from Escherichia coli O111:B4;100 ng/mL; Invivogen) for 4 hours.

    Magnetic Cell Separation:

    Article Title: HIVEP1 Is a Negative Regulator of NF-κB That Inhibits Systemic Inflammation in Sepsis.
    Article Snippet: Buffy coats were collected from heparin anticoagulated blood by density-gradient centrifugation using Ficoll-paque plus (GE Healthcare, Stockholm, Sweden). .. Subsequently, monocytes were isolated using cluster-of-differentiation (CD)14+ magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany, # 130-050- 201) and MACS LS columns (Miltenyl Biotech). .. For stimulations, monocytes were placed in a 48-well plate (106 per well; Greiner Bio-One, Frickenhausen, Germany) in RPMI1640 medium containing 10% fetal calf serum (FCS),100 U/ml penicillin, 2 mM L-glutamine and stimulated with medium, Pam3CSK4 (1 μg/mL; Invivogen, San Diego, CA) or LPS (from Escherichia coli O111:B4;100 ng/mL; Invivogen) for 4 hours.

    Article Title: HIVEP1 Is a Negative Regulator of NF-κB That Inhibits Systemic Inflammation in Sepsis
    Article Snippet: Buffy coats were collected from heparin anticoagulated blood by density-gradient centrifugation using Ficoll-paque plus (GE Healthcare, Stockholm, Sweden). .. Subsequently, monocytes were isolated using cluster-of-differentiation (CD)14 + magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany, # 130-050-201) and MACS LS columns (Miltenyl Biotech). .. For stimulations, monocytes were placed in a 48-well plate (10 6 per well; Greiner Bio-One, Frickenhausen, Germany) in RPMI1640 medium containing 10% fetal calf serum (FCS),100 U/ml penicillin, 2 mM L-glutamine and stimulated with medium, Pam3CSK4 (1 μg/mL; Invivogen, San Diego, CA) or LPS (from Escherichia coli O111:B4;100 ng/mL; Invivogen) for 4 hours.



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